The Naperville Heritage Society's permanent museum collection includes nearly 80,000 two- and three-dimensional items including:
- Household and industrial objects
- Furniture, clothing, textiles, paintings, sculptures, architectural fragments, transportation artifacts and agricultural implements
- Prints, photographs, films, audio / video tapes and multimedia
- Business, institutional and personal archives, such as original manuscripts and documents, including diaries, letters, ledgers and journals
The majority of the museum's three-dimensional artifacts are displayed in settings at Naper Settlement that depict their original use.